Regulatory
Gal4-UAS

Part:BBa_K1884004:Design

Designed by: Chengrong Xie   Group: iGEM16_SZU-China   (2016-10-07)


Upstream activating sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We gain this part from Shenzhen Engineering Laboratory of Marine Algal Biotechnology

References

[1]Webster, Nocholas; Jin, Jia Rui; Green, Stephen; Hollis, Melvyn; Chambon, Pierre (29 January 1988). "The Yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator". Cell. 52 (2): 169–178. doi:10.1016/0092-8674(88)90505-3. PMID 2830022.

[2]West, Jr., Robert W.; Yocum, R. Rogers; Ptashne, Mark (November 1984). "Saccharomyces cerevisiae GAL1-GAL10 Divergenet Promoter Region: Location and Function of the Upstream Activating Sequence UASG". Molecular and Cellular Biology. 4 (11): 2467–2478.

[3]Lewandoski, Mark (October 2001). "Conditional control of gene expression in the mouse". Nature Reviews Genetics. 2: 743–755. doi:10.1038/35093537.

[4]Wion, Didier; Casadesus, Josep (March 2006). "N6-methyl-adenine: An epigenetic signal for DNA-protein interactions". Nature Reviews Microbiology. 4: 183–192. doi:10.1038/nrmicro1350